BEGIN:VCALENDAR VERSION:2.0 PRODID:-//ChamberMaster//Event Calendar 2.0//EN METHOD:PUBLISH X-PUBLISHED-TTL:P1H REFRESH-INTERVAL:P1H CALSCALE:GREGORIAN BEGIN:VTIMEZONE TZID:America/New_York BEGIN:DAYLIGHT RRULE:FREQ=YEARLY;BYMONTH=3;BYDAY=2SU DTSTART:20070101T000000 TZOFFSETFROM:-0500 TZOFFSETTO:-0400 TZNAME:Eastern Daylight Time END:DAYLIGHT BEGIN:STANDARD RRULE:FREQ=YEARLY;BYMONTH=11;BYDAY=1SU DTSTART:20070101T000000 TZOFFSETFROM:-0400 TZOFFSETTO:-0500 TZNAME:Eastern Standard Time END:STANDARD END:VTIMEZONE BEGIN:VEVENT DTSTART;TZID=America/New_York:20260915T120000 DTEND;TZID=America/New_York:20260915T130000 X-MICROSOFT-CDO-ALLDAYEVENT:FALSE SUMMARY:CYTO U Webinar: Emerging lab-on-a-particle technologies for functional single-cell analysis DESCRIPTION:Learn More and Register: ISAC Learning: Emerging lab-on-a-particle technologies for functional single-cell analysisCompartmentalizing single cells at the microscale is crucial for screening heterogeneous populations or libraries of mutants for functional properties such as secretion\, cell-cell interactions or growth. Example applications include T cell receptor and antibody discovery as well as microbial colony picking\, which have significant implications from drug screening to therapeutic development. Technologies based on microfluidics such as microwell and microdroplets have been applied to these problems. However\, while these tools have been demonstrated to effectively perform cell assays\, they typically require specialized instrumentation and are either limited in throughput or transport required for extended cell culture and reagent exchange\, hampering wide adoption. More recently\, lab-on-a-particle technologies have emerged as complementary tools for carrying out microscale reactions to analyze molecules and cells in a high-throughput manner. Microparticles suspended in oil-free aqueous phase and compatible with conventional laboratory tools such as microscopy and flow cytometry can be leveraged for assaying various functional cell assays. In this webinar\, two implementations of lab-on-a-particle technologies will be introduced\, namely (1) nanovials and (2) capped nanvoials. First\, nanovials are cavity-containing particles that facilitate compartmentalization of single cells and capture of secreted proteins on the surface of the cavity. Here\, nanovials are used to assay the single-cell secretion phenotype of collagen\, a critical extracellular matrix (ECM) protein also known to play a role in fibrosis. Second\, capped nanovials\, which are self-assembling micro-compartments formed by "capping" nanovials with spherical particles\, are introduced as a novel microcompartment functionally akin to a test tube. Crucially\, capped nanovials are formed in a massively parallel manner ( 100\,000 capped nanovials/mL prepared in ~10 minutes) by simple pipetting and centrifugation and analyzed using accessible instruments such as microscopes and flow cytometers. Using this platform\, key biomedical applications including yeast colony growth assay and a paired antibody-secreting and reporter cell assay are demonstrated.The Speaker\nYuta Nakagawa is a project assistant professor at The University of Tokyo\, where his research focuses on developing novel flow cytometry tools to enable single-cell screening based on their function including secretion\, proliferation\, and cell-cell communication. He received his PhD from The University of Tokyo under the mentorship of Professor Keisuke Goda\, where he worked on developing cutting-edge flow cytometers such as a high-throughput microfluidic droplet sorter. He was then trained as a postdoctoral scholar at UCLA under Professor Dino Di Carlo\, where he worked on developing novel functional assays leveraging microparticles and flow cytometry. He is actively engaged with the ISAC community\, including attendance at CYTO conferences and membership of Marylou Inghram Scholar of the ISAC leadership development program. X-ALT-DESC;FMTTYPE=text/html:

Learn More and Register: ISAC Learning: Emerging lab-on-a-particle technologies for functional single-cell analysis

Compartmentalizing single cells at the microscale is crucial for screening heterogeneous populations or libraries of mutants for functional properties such as secretion\, cell-cell interactions or growth. Example applications include T cell receptor and antibody discovery as well as microbial colony picking\, which have significant implications from drug screening to therapeutic development. Technologies based on microfluidics such as microwell and microdroplets have been applied to these problems. However\, while these tools have been demonstrated to effectively perform cell assays\, they typically require specialized instrumentation and are either limited in throughput or transport required for extended cell culture and reagent exchange\, hampering wide adoption. More recently\, lab-on-a-particle technologies have emerged as complementary tools for carrying out microscale reactions to analyze molecules and cells in a high-throughput manner. Microparticles suspended in oil-free aqueous phase and compatible with conventional laboratory tools such as microscopy and flow cytometry can be leveraged for assaying various functional cell assays. In this webinar\, two implementations of lab-on-a-particle technologies will be introduced\, namely (1) nanovials and (2) capped nanvoials. First\, nanovials are cavity-containing particles that facilitate compartmentalization of single cells and capture of secreted proteins on the surface of the cavity. Here\, nanovials are used to assay the single-cell secretion phenotype of collagen\, a critical extracellular matrix (ECM) protein also known to play a role in fibrosis. Second\, capped nanovials\, which are self-assembling micro-compartments formed by &ldquo\;capping&rdquo\; nanovials with spherical particles\, are introduced as a novel microcompartment functionally akin to a test tube. Crucially\, capped nanovials are formed in a massively parallel manner (>\;100\,000 capped nanovials/mL prepared in ~10 minutes) by simple pipetting and centrifugation and analyzed using accessible instruments such as microscopes and flow cytometers. Using this platform\, key biomedical applications including yeast colony growth assay and a paired antibody-secreting and reporter cell assay are demonstrated.

The Speaker
Yuta Nakagawa \;is a project assistant professor at The University of Tokyo\, where his research focuses on developing novel flow cytometry tools to enable single-cell screening based on their function including secretion\, proliferation\, and cell-cell communication. He received his PhD from The University of Tokyo under the mentorship of Professor Keisuke Goda\, where he worked on developing cutting-edge flow cytometers such as a high-throughput microfluidic droplet sorter. He was then trained as a postdoctoral scholar at UCLA under Professor Dino Di Carlo\, where he worked on developing novel functional assays leveraging microparticles and flow cytometry. He is actively engaged with the ISAC community\, including attendance at CYTO conferences and membership of Marylou Inghram Scholar of the ISAC leadership development program.

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